miR-7 AND miR-34a SEQUENCE CLONING AND EXPRESSION IN A1235 GLIOBLASTOMA CELL LINE

Dora Kolic; Luka Horvat; Maja  Setinc; Mariastefania Antica; Maja Matulic

doi:10.33602/mebm.3.2.4

Dora Kolic Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Luka Horvat Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Maja Setinc Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Mariastefania Antica Division of Molecular Biology, Rudjer Boskovic Institute, Zagreb, Croatia
Maja Matulic Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia

DOI: https://doi.org/10.33602/mebm.3.2.4

Keywords: miR-7, miR-34a, expression, glioblastoma cell line

Abstract

miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.