INHIBITION OF PARP ACTIVITY DOES NOT AFFECT THE DIFFERENTIATION PROCESSES CAUSED BY RETINOIC ACID IN SH-SY5Y CELLS

Luka Horvat; Martina Grubar; Josip Madunic; Mariastefania Antica; Maja Matulic

doi:10.33602/mebm.2.1.7

Luka Horvat Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Martina Grubar Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Josip Madunic Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Mariastefania Antica Division of Molecular Biology, Rudjer Boskovic Institute, Zagreb, Croatia
Maja Matulic Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia

DOI: https://doi.org/10.33602/mebm.2.1.7

Keywords: neuroblastoma cell line, differentiation, retinoic acid, PARP1

Abstract

Differentiation is a complex process by which cells become specialized in their physiological functions. As this process is followed by a decrease in proliferation ability, induction of differentiation could be an ideal cancer treatment. We analysed the effects of a common differentiation agent, retinoic acid on neuroblastoma SH-SY5Y cells under the conditions of poly(ADP-ribose) polymerase (PARP) inhibition. Namely, PARP1 can be indirectly involved in processes of chromatin remodelling. Cells differentiated after retinoic acid treatment into neural cells, with neurite outgrowth, proliferation arrest and induction of tissue plasminogen activator. PARP inhibition did not influence the process of differentiation. Analysis of gene expression revealed the involvement of TGF β, Notch and pathways related to epithelial-mesenchymal transition to be involved in RA-dependent differentiation.