THE ASSOCIATION OF VIABLE MONONUCLEAR CELLS AND CFU-GM WITH TIME OF HEMATOPOIETIC ENGRAFTMENT

Abstract

The quality of the collected haematopoietic stem cells (HSC) product is of primary importance for each patient in the transplantation process. We have collected data on the number and viability of cells in the HSC engraftment, both before and after freezing, and compared it to the rate of hematologic recovery to evaluate and to determine the laboratory parameter that sufficiently determines the rate of recovery after transplantation. We concluded that the average percentage of viability and the average stem cell count per kg of patient was consistent with the recommended values; the number of CFU-GM in the fresh leukapheric sample was associated with the rate of neutrophil recovery, whereas no statistical significance was found in association with other measurements, and the cell viability and growth of CFU-GM in this study did not show a direct impact on patient recovery rate.